630 Microbiology for Teachers

Bacterial Genetics

Mutation

• Mutations

   

• Molecular basis of mutation
  • Base-pair substitution

   

  • Frameshift mutations

   

  • Reversion

   

• Mutation rates
  • Missense mutations

   

  • Nonsense mutations

   

  • Transpositions

   

• Mutagens

   

  • Base analogs

   

  • UV or X-irradiation

   

  • DNA repair errors

   

  • Transposons

   

• Ames test

   

Genetic Recombination

Genetic Recombination is genetic exchange between homologous nucleic acid sequences from different sources; accomplished by bacteria in several ways:

• Transformation

   

   

• Transduction

   

   

   

• Conjugation

   

   

   

Transposition

• Transposable elements

   

• Gene function is disrupted

   

• Other genes may also be carried and inserted

   

• Transposition occurs at frequencies of 10^-5 to 10^-7 per generation